Bacterial Synthesis of Nucleotides

Enzymatic Synthesis of the Peptide in Bacterial Uridine Nucleotides

The pentapeptide moiety of uridine diphosphate acetylmuramyl-pentapeptide, a precursor of the peptidoglycan of bacterial cell walls, is synthesized in a reaction sequence involving five enzymes. The amount and specificity of these enzymes have been studied in 10 microorganisms, five of which contain L-lysine as the third amino acid in the peptide sequence, and five of which contain instead meso...

Enzymatic synthesis of purine nucleotides.

The demonstration of a pathway of pyrimidine nucleotide synthesis in which PRPP’ is a key intermediate (1, 2) prompted a study to determine whether the same type of reaction mechanism might apply to the synthesis of purine nucleotides. In a preliminary note the condensation of adenine with PRPP to yield A5P was reported (3). Subsequent work has established the existence of enzymes in yeast whic...

Pyrrolopyrimidine nucleotides and protein synthesis.

The interaction of amino pyrrolopyrimidine nucleotides with enzymes catalyzing different steps in the reaction sequence leading to the incorporation of amino acids into polypeptides has been studied. None of the analogue nucleotides could function as the energy source for activation of amino acids or esterification of transfer RNA (tRNA) by mammalian or bacterial enzymes. Toyocamycin S’-triphos...

Synthesis in the study of nucleotides.

Enzymatic Synthesis of the Peptide in Bacterial Uridine Nucleotides. Iv. Purification and Properties of D-glutamic Acid-adding Enzyme.

The pentapeptide moiety of uridine diphosphate acetylmuramyl-pentapeptide, a precursor of the peptidoglycan of bacterial cell walls, is synthesized in a reaction sequence involving five enzymes. The amount and specificity of these enzymes have been studied in 10 microorganisms, five of which contain L-lysine as the third amino acid in the peptide sequence, and five of which contain instead meso...